Abstract

In the present study, copper oxide nanoparticles (CuO NPs) were synthesized using a eco-friendly
technique and evaluated their antibacterial activity. At the first time, Brassica tournefortii leaves
extract mediated copper oxide-based nanoparticles which prepared by a bio-reduction method
using an aqueous Brassica tournefortii leaves extract as both a reducing and stabilizing agent.
Copper oxide nanoparticles were characterized by using ultraviolet-visible spectroscopy (UV-VIS),
Fourier transform-infrared spectroscopy (FT-IR), x-ray diffraction (XRD) and scanning electron
microscope (SEM) techniques. The synthesized CuO nanoparticles were also evaluated for their
antibacterial activity against gram-positive (Staphylococcus aureus and Streptococcus spp) and
gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial strains. UV-Vis and FT-IR
results showed that the successful formation and stabilization of copper oxide nanoparticles due
to biomolecules in Brassica tournefortii extract. XRD results indicated that the CuO nanoparticles
were monoclinic of size 27.73 nm. SEM image showed that CuO nanoparticles had spherical
morphology. Furthermore, Antibacterial activity showed that CuO nanoparticles have good
antibacterial agent against both gram positive and gram-negative organisms. The antibacterial
assay revealed that Pseudomonas aeruginosa a maximum zone of inhibition (44 mm) at 30 mg/ml concentration of CuO nanoparticles.